Journal: International Journal of Molecular Sciences

Article Title: Radiofrequency Treatment Attenuates Age-Related Changes in Dermal–Epidermal Junctions of Animal Skin

doi: 10.3390/ijms25105178

Figure Lengend Snippet: Expression of HSP47, HSP90, TGF-β, and DEJ proteins following RF treatment in senescent keratinocytes. ( A ) Schematic representation of non-senescent keratinocytes, senescent keratinocytes, and RF treatments administered to senescent keratinocytes. ( B – H ) Proteins were extracted from senescent keratinocytes following RF treatment and subjected to the following assays. ( B – D ) Western blot analysis of HSP47 and HSP90. ( E ) ELISA results for TGF-β. ( F , G ) Western blot analysis of collagen XVII. ( H ) ELISA results for collagen IV. Data are presented as mean ± SD of three independent experiments. **, p < 0.01 vs. first bar; $$, p < 0.01 vs. second bar (Mann–Whitney U test). CON, control; DEJ, dermal–epidermal junction; ELISA, enzyme-linked immunosorbent assay; GM, growth medium; HSP47, heat shock protein 47; HSP90, heat shock protein 90; MW, molecular weight; PBS, phosphate-buffered saline; RF, radiofrequency; SD, standard deviation; SnCs, senescent; TGF-β, transforming growth factor beta.

Article Snippet: Primary human epidermal keratinocytes (HEKn; American Type Culture Collection [ATCC], Manassas, VA, USA) were cultured in growth medium (GM) composed of dermal cell basal medium (ATCC) supplemented with a keratinocyte growth kit (ATCC).

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Control, Molecular Weight, Saline, Standard Deviation

Journal: Cell reports

Article Title: Vimentin modulates regulatory T cell receptor-ligand interactions at distal pole complex, leading to dysregulated host response to viral pneumonia

doi: 10.1016/j.celrep.2024.115056

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse Keratinocyte Growth Factor, KGF/FGF-7 ELISA Kit , MyBioSource.com , Cat#MBS701004.

Techniques: Purification, Blocking Assay, Plasmid Preparation, Functional Assay, Control, Virus, Recombinant, Avidin-Biotin Assay, Cell Isolation, Enzyme-linked Immunosorbent Assay, Software

Journal: Gels

Article Title: Arginine-Biofunctionalized Ternary Hydrogel Scaffolds of Carboxymethyl Cellulose–Chitosan–Polyvinyl Alcohol to Deliver Cell Therapy for Wound Healing

doi: 10.3390/gels10110679

Figure Lengend Snippet: ( A ) An illustrative representation of a cross-section of the skin, indicating the layers (epidermis and dermis) that were resembled by the 3D hybrid hydrogel, depicted with information regarding its components, chemical formula, and structure. ( B ) Scheme of the co-culture strategy of the cell therapy product simulating the skin layers, with the deposition of keratinocytes in the membrane layer (resembling basement membrane of skin) and populating the porous layer with fibroblasts to obtain a cellular biomimetic dermal–epidermal skin substitute construct (not to scale).

Article Snippet: For keratinocytes, Gold Keratinocyte Growth Basal Medium supplemented with a KGM-Gold TM Keratinocyte Single Quots TM Kit (Lonza, Morristown, NJ, USA), referred to as “complete basal keratinocyte medium”, was used.

Techniques: Co-Culture Assay, Membrane, Construct

Journal: Gels

Article Title: Arginine-Biofunctionalized Ternary Hydrogel Scaffolds of Carboxymethyl Cellulose–Chitosan–Polyvinyl Alcohol to Deliver Cell Therapy for Wound Healing

doi: 10.3390/gels10110679

Figure Lengend Snippet: ( A ) Results of cell viability (MTT cytotoxicity test) and ( B ) cell proliferation (measured using resazurin protocol) biological assays for the porous three-dimensional structures (3d = 3 days). ( C ) Calcein AM (viable cells, green fluorescence, left side images, identified as LIVE) and ethidium homodimer (dead cells, red fluorescence, right side images, identified as DEAD) staining for keratinocyte and fibroblast monoculture cells deposited onto the lower and upper faces of the L-arginine functionalized 3D constructs, respectively (scale bar = 100 µm). Details: schematic representation of cell deposition (not to scale).

Article Snippet: For keratinocytes, Gold Keratinocyte Growth Basal Medium supplemented with a KGM-Gold TM Keratinocyte Single Quots TM Kit (Lonza, Morristown, NJ, USA), referred to as “complete basal keratinocyte medium”, was used.

Techniques: Fluorescence, Staining, Construct

Journal: Gels

Article Title: Arginine-Biofunctionalized Ternary Hydrogel Scaffolds of Carboxymethyl Cellulose–Chitosan–Polyvinyl Alcohol to Deliver Cell Therapy for Wound Healing

doi: 10.3390/gels10110679

Figure Lengend Snippet: Fluorescence microscopy images showing representative images of the 3D CMC:PVA:CHI_L-Arg structure, where a co-culture of ( C ) keratinocytes (upper face—membrane) and ( D , E ) fibroblasts (lower face—porous structure) were seeded compared to controls (( A ) Keratinocyte +Control and ( B ) Fibroblast +Control). Cells were labeled with calcein AM (viable cells, green fluorescence, left column, identified as LIVE) and ethidium homodimer (dead cells, red fluorescence, right column, identified as DEAD). Scale bar = 100 µm. Detail: schematic representation of cell therapy dermal–epidermal skin substitute (not to scale).

Article Snippet: For keratinocytes, Gold Keratinocyte Growth Basal Medium supplemented with a KGM-Gold TM Keratinocyte Single Quots TM Kit (Lonza, Morristown, NJ, USA), referred to as “complete basal keratinocyte medium”, was used.

Techniques: Fluorescence, Microscopy, Co-Culture Assay, Membrane, Control, Labeling

Journal: Gels

Article Title: Arginine-Biofunctionalized Ternary Hydrogel Scaffolds of Carboxymethyl Cellulose–Chitosan–Polyvinyl Alcohol to Deliver Cell Therapy for Wound Healing

doi: 10.3390/gels10110679

Figure Lengend Snippet: Histopathological analysis of skin lesions in Hairless mice, showing representative images of ( A ) skin from the control group (no hydrogel applied) and ( B ) skin from the group treated with the 3D bilayer construct containing fibroblasts and keratinocytes (CM group). Black asterisk: intact epidermis; red asterisk: denser collagen; green asterisk: looser collagen; black arrow: reactive fibroblasts; red arrow: blood capillaries. The larger image displays the lesion area at 20× magnification, with a zoomed-in view of the highlighted area at 40× magnification.

Article Snippet: For keratinocytes, Gold Keratinocyte Growth Basal Medium supplemented with a KGM-Gold TM Keratinocyte Single Quots TM Kit (Lonza, Morristown, NJ, USA), referred to as “complete basal keratinocyte medium”, was used.

Techniques: Control, Construct